Making And Using Visible Absorption Measurements

INTRODUCTION
      The main theory behind this experiment is that by development a spectrophotometer the visible spectrum of an absorbing species can be secureed. use these findings the uninflected wavelength can be determine. By collecting the absorbance selective information a Beers Law plot can be constructed. Using this plot the concentration of the absorbing species in a upshot containing an unknown region concentration of the species can be determined. The equations disposed(p) to this experiment include:
(1).%T=(It/Io)(100) (2). Log (%T/100)= Log(It/Io) (3). A= Log(It/Io)

(4). A= Log (%T/100)
This experiment relates to what is being knowledgeable by showing how an absorption species can be finded and ascertain the concentrations for such species
OBJECTIVE
      The objective of this experiment is to use a spectrophotometer to obtain the visible spectrum of an absorbing species. Another objective is to determine the analytical wavelength, the absorption, and the percent transmittance and use this data to create a Beers Law plot.




MATERIALS AND EQUIPMENT
The materials required for this experiment include the quest:
Distilled water (as needed)
Spectrophotometer
About 8 cuvets
Pipet
50 mL graduated cylinder
PROCEDURE
Six standard solutions and one unknown solutions were prepared. The concentrations of the solutions were recorded in the data table. The spectrophotometer was then turned on and adjusted until displayed 0%T. Distilled water was to rinse out the cuvet. The cuvet was filled about half way and then placed into the spectrophotometer. This step was performed for each of the solutions. The wavelength curb knob was adjusted according to the analytical wavelength for the assigned species. To obtain the absorbance of the solutions, the cuvet was rinsed three times with a small portion of the unknown solution. The cuvet was then filled half way with the unknown solution and the absorbance was determined. When the determinations...If you want to get a full essay, order it on our website: Ordercustompaper.com



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